1.1.1.22 ADP - 13 1.1.1.22 AMP - 30 1.1.1.22 Ca2+ 84% residual activity at 1 mM 15 1.1.1.22 EDTA 83% residual activity at 1 mM 21 1.1.1.22 gallic acid is a non-competitive inhibitor with respect to UDP-glucose and NAD+. It decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Gallic acid exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of gallic acid, the activity remaining after 30 min is 55% that of control 764 1.1.1.22 Mn2+ 84% residual activity at 1 mM 11 1.1.1.22 additional information no substrate inhibition, K+ is not inhibitory up to 500 mM, not inhibitory: dimethyldicarbonate, EDTA 2 1.1.1.22 additional information in the presence of 0.12 mM 5-azido-UDP-glucose, 89% of the enzyme activity is lost after 5 min of photolabeling. When the enzyme is photolyzed in presence of 1 mM UDP-glucose, 11% of the enzyme activity is lost. 5-azido-UDP-glucose is photoinserting into a UDP-glucose-binding site on the human enzyme in a specific manner. Uracil, uridine, and glucose have a poor protective effect on the labeling, while UDP and UDP-glucose strongly inhibit photoinsertion 2 1.1.1.22 additional information inhibition of human UDP-glucose dehydrogenase expression using siRNA expression vector in breast cancer cells 2 1.1.1.22 additional information the enzyme from serotype K30 is not inhibited by NAD+, in contrast to Escherichia coli K-12 2 1.1.1.22 additional information enzyme is active in the absence of metal ions 2 1.1.1.22 NaCl VNG1048G loses much of its activity when salinity drops below 3 M NaCl 42 1.1.1.22 NAD+ substrate inhibition, reversible by the addition of a nucleotide triphosphate, e.g. ATP, in the absence of kinase. Mutations in a previously identified UDP-glucuronic acid allosteric binding site decrease the binding affinity of the nucleotide triphosphate 7 1.1.1.22 NADH - 8 1.1.1.22 NADH 0.1 mM, presence of 0.1 mM NAD+, 44% inhibition, presence of 0.5 mM NAD+, 6% inhibition 8 1.1.1.22 NADH product inhibition, 26% residual activity at 0.05 mM; product inhibition, 70% residual activity at 0.05 mM 8 1.1.1.22 NADH 0.05 mM, partial inhibition 8 1.1.1.22 NH2OH deactivation 1109 1.1.1.22 p-chloromercuribenzoate - 43 1.1.1.22 p-chloromercuribenzoate 0.001 mM, 66% inhibition 43 1.1.1.22 phycocyanin mixed-type inhibitor with respect to both UDP-glucose and NAD+ 256600 1.1.1.22 phycocyanobilin competitive with respect to UDP-glucose and non-competitive with respect to NAD+. Phycocyanobilin is also effective in reducing the colony formation capacity of PC-3 prostate cancer cells and FTC-133 thyroid cancer cells 47548 1.1.1.22 piperine stronger inhibition with intestinal cell enzyme than with rat liver enzyme 20806 1.1.1.22 piperine non-competitive, reversible, non-pH-dependent, 90% inhibition at concentration 0.05 mM and 10% inhibition at concentration 0.01 mM 20806 1.1.1.22 quercetin shows a competitive inhibition and a mixed-type inhibition with respect to UDP-glucose and NAD+, decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Quercetin exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of quercetin, the activity remaining after 30 min is 20% that of control 137 1.1.1.22 SDS strong inhibition 124 1.1.1.22 thiol group modifying reagents - 116881 1.1.1.22 UDP 0.5 mM, partial inhibition 26 1.1.1.22 UDP-alpha-D-glucuronate 0.05 mM, partial inhibition 995 1.1.1.22 UDP-alpha-D-glucuronate slight product inhibition 995 1.1.1.22 UDP-alpha-D-xylose competitive. The DELTA132 deletion mutant and the UDP-alpha-D-xylose-inhibited structures have similar hexamer-building interfaces, suggesting that the hinge-bending motion represents a path for the allosteric transition between the different hexameric states 2438 1.1.1.22 UDP-D-galactose slight 1336 1.1.1.22 UDP-D-xylose - 341 1.1.1.22 UDP-D-xylose competitive with UDPglucose 341 1.1.1.22 UDP-galactose 0.5 mM, partial inhibition 129 1.1.1.22 UDP-glucuronate competitive 226 1.1.1.22 UDP-glucuronic acid product inhibition, 44% residual activity at 1 mM; product inhibition, 79% residual activity at 1 mM 1225 1.1.1.22 UDP-xylose competitive 1013 1.1.1.22 UDP-xylose - 1013 1.1.1.22 UDP-xylose 0.05 mM, partial inhibition 1013 1.1.1.22 UDP-xylose a potent UGDH inhibitor 1013 1.1.1.22 UDP-xylose allosteric feedback inhibitor, reduces significantly the activity of the enzyme 1013 1.1.1.22 UDParabinose - 44919 1.1.1.22 UDPgalacturonic acid - 12723 1.1.1.22 UDPglucuronate - 630 1.1.1.22 UDPglucuronate product feed-back inhibition competitive with UDPglucose 630 1.1.1.22 UDPxylose - 4576 1.1.1.22 Urea strong inhibition 116 1.1.1.22 Uridine 5'-diphosphate chloroacetol alkylates a thiol group of cysteine in the catalytic centre via being specifically bound instead of the substrate 44922 1.1.1.22 Zn2+ strong inhibition 14 1.1.1.22 Zn2+ the presence of Zn2+ ions markedly stimulates catalytic activity 14