4.1.2.32 enzyme is unstable when purified more than about 5-fold 5133 4.1.2.32 exposure of the enzyme for 30 min at 0°C to buffers of pH values in the region 4 to 9 does not result in loss of activity followed by adjustment of the pH 6.0 5133 4.1.2.32 inclusion of mercaptoethanol, EDTA and PMSF in the preparative buffers enhance yield and stability of microsomal activity 5136 4.1.2.32 trimethylamine-grown Pseudomonas aminovorans enzyme is more labile at 40°C than enzyme from methylamine-grown bacteria in French press extracts 5133