3.5.4.5 irreversible denaturation after 30 min at 72°C 657220 3.5.4.5 irreversible denaturation at 72°C after 30 min 657220 3.5.4.5 unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions 209652 3.5.4.5 very unstable 209656