2.7.4.14 bovine serum albumin, 0.1 mg/ml, is completely effective in preventing the loss of activity in the dilute preparation 645137 2.7.4.14 dialysis against 20 mM phosphate, 1 mM MgCl2, 20% ethylene glycol, pH 8.0, 90% loss of activity 645147 2.7.4.14 freeze-thawing inactivates 645147 2.7.4.14 more stable in histidine than in phosphate buffer 645141 2.7.4.14 the enzyme is unstable when fully activated, anions promoting hydrophobic interactions stabilize the active conformation 645142 2.7.4.14 the purified enzyme is notably unstable 645141 2.7.4.14 three-fold dilution of a preparation of 0.2 mg of protein per ml results in less of 50% of the activity in 1 h 645137, 645140