1.5.1.25 irreversible inactivation at protein concentration below 1 mg/ml. Complete loss of activity after 24 h at 4°C. Higher protein concentrations or 10% glycerol prevent enzyme inactivation. 30% loss of activity aftter 7 days at 4°C 440358 1.5.1.25 the purified recombinant enzyme is unstable even in the presence of substrates and requires factors to stabilize it, e.g. 20% glycerol and 1 mM dithiothreitol (DTT) 743355