1.10.3.3 20-25% retention of activity after immobilization, at 12°C, stable for at least 30 days 439919 1.10.3.3 a long acting enzyme derivative is synthesized by covalently linking poly(ethylene glycol) to the enzyme 696121 1.10.3.3 ascorbate oxidase is alternately deposited with Au nanoparticles and forms a layer-by-layer membrane on a Pt electrode. The enzyme activities remain even after the deposition. The oxidation current of ascorbic acid (0.1 mM) decreases to 36% for the (ascorbate oxidase/Au)1 modified electrode, and to 19% for the (ascorbate oxidase/Au)10 modified electrode 690102 1.10.3.3 gelatin, catalase, peroxidase and methemoglobin protect against inactivation 439889 1.10.3.3 immobilization within 6% Ca-alginate gel beads improves stability 439911 1.10.3.3 no change in total activity in the presence of 5 M urea, conversion of tetrameric form into monomer with 75% activity in 8 M urea 439924 1.10.3.3 partially unfolding of the enzyme by 1.4 M guanidinium hydrochloride or 2.8 M urea 673569 1.10.3.3 role of copper in stability 439912 1.10.3.3 stable to dialysis against EDTA or cyanide 396331