4.2.1.65 not stabilized by rapid freezing in liquid nitrogen, storage at 4 or -20ŚC with mercaptoethanol, dithiothreitol, beta-cyano-L-alanine or asparagine 4.2.1.65 stabilized as ammonium sulfate precipitate 4.2.1.65 very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect