2.7.1.77 dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity 641990 2.7.1.77 does not lose acttivity on lyophilization 641993 2.7.1.77 no loss of activity after dialysis overnight against distilled water 641990 2.7.1.77 not stable to SDS 641993 2.7.1.77 stable in presence of 2 M urea, but not 4 M urea 641993 2.7.1.77 storage in 20% acetone for 1 week without loss of activity 641996 2.7.1.77 the enzyme can be isolated and handled only in the presence of detergents 641993 2.7.1.77 the enzyme tolerates repeated freeze-thawing 642001 2.7.1.77 tolerates repeated freezing and thawing 641993 2.7.1.77 withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml 641994