2.6.1.2	during the purification procedure the enzyme needs protective substances, i.e. glycerol, 2-mercaptoethanol and alanine	
2.6.1.2	uneffected by repeated freezing and thawing	
2.6.1.2	enzyme does not tolerate freezing at any stage after sonication of the isolated mitochondria	
2.6.1.2	K+ or L-alanine stabilizes	
2.6.1.2	pyridoxal 5'-phosphate, L-alanine or 2-oxoglutarate protects against heat inactivation