1.8.2.1 catalytic asctivity is retained upon immobilization of the enzyme on an edge oriented pyrolytic graphite working electrode 1.8.2.1 maturation of enzyme requires the combined function of the two apocytochrome c chaperones CcmI and SirG 1.8.2.1 proteolytic treatment with trypsin at room temperature for up to 5 h at trypsin to enzyme ratios of 1/20 and 1/1 does not affect the activity of the enzyme 1.8.2.1 stable to repeated freezing and thawing 1.8.2.1 thawing and refreezing has no effect 1.8.2.1 treatment with 1-2 mg trypsin per 1.5 mg of protein or 0.1-0.2 mg of pronase or 1.5 mg of protein at 25°C causes decline of activity