1.4.1.13 2 mM alpha-ketoglutarate, 10 mM beta-mercaptoethanol and 10% glycerol when present together markedly enhance stability 391440 1.4.1.13 2-mercaptoethanol, stabilizes 391438, 391455 1.4.1.13 alpha-ketoglutarate stabilizes 391438, 391447, 391448 1.4.1.13 alpha-ketoglutarate, L-glutamine, L-glutamate and NADP when present together partial protect against enzyme inactivation 391438 1.4.1.13 DTT, 1 mM, stabilizes at 4°C 391455 1.4.1.13 EDTA, stabilizes 391448, 391455 1.4.1.13 glutamine stabilizes 391447, 391448 1.4.1.13 incubation with L-glutamine or L-glutamate results in marked time- and temperature-dependent loss in activity 391438 1.4.1.13 NADP causes relatively small loss in activity compared with control values 391438 1.4.1.13 unprotected enzyme is unstable, losing 65% of its activity in 24 hours at 4°C 391440