1.13.11.60 8R-dioxygenase is unstable during purification. It can be stabilized by glutathione, glutathione peroxidase and ethylenediaminetetraacetic acid 1.13.11.60 repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme 1.13.11.60 the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity