2.5.1.20	2-mercaptoethanol	10 mM, not as effective as dithiothreitol	638726	
2.5.1.20	dithiothreitol	10 mM, required for maximal stability	638726	
2.5.1.20	EDTA	approx. 3.5fold activation at 20 mM	638736	
2.5.1.20	glutathione	required for maximal activity	638730, 638731	
2.5.1.20	additional information	recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity, which seem to be contained in latex species	723380	
2.5.1.20	neryl diphosphate	accelerates activity	638725	
2.5.1.20	Rubber elongation factor	amino acid sequence of REF	638729	
2.5.1.20	Rubber elongation factor	i.e.REF, protein tightly bound to serum-free rubber particles, required for prenyltransferase activity, no requirement for farnesyl diphosphate synthase activity	638728	
2.5.1.20	rubber particle	-	638730	
2.5.1.20	sulfhydryl compound	essential for full activity	638725