2.5.1.16 glycerol expression of SPDS is barely detectable in the callus grown on callus growth medium (composed of basal Murashige and Tucker salts), which shows steady increase on embryo-inducing medium including 2% glycerol until the end of culture at day 40. Expression level of the gene is induced by glycerol from globular embryos to cotyledonary-stage embryos to a different extent, suggesting that the polyamine biosynthetic genes are related to somatic embryogenesis 693859 2.5.1.16 additional information increased enzyme activity after wounding of the potatoe 700713