2.4.1.96	additional information	enzyme appears to exist as an inactive proenzyme which can be activated by incubation of crude cell extracts with endogenous or exogenous proteases	489625	
2.4.1.96	additional information	proteolytic activation mechanism might be the first step in the activation of IFP-synthase after cell shrinkage due to osmotic water loss. About 1 h later when proteolytic activation is no longer evident and the isofloridoside pool is almost filled up a second maximum of active IFP-synthase is evident. This type of enzyme exhibits different molecular properties and might by involved in the turnover of the isofloridoside pool	489624