2.3.1.241 Triton X-100 greatly enhances the activity of the enzyme at lower concentrations, but the activity declines only gradually as the concentration of detergent increases 726949 2.3.1.241 Triton X-100 in the presence of 1 or 5 mM MgCl2, the enzyme shows a dependence on added detergen. With Triton X-100, the highest activity is observed between 0.1 and 0.2% (w/v). At higher Triton X-100 concentrations, the activity dropps off, presumably because of surface dilution of the substrate in mixed micelles. At 5 mM MgCl2, the Triton X-100 optimum is considerably broader 727818