2.1.1.229 TRM112 interaction of ALKBH8 with a small accessory protein, TRM112, is required to form a functional tRNA methyltransferase 713016 2.1.1.229 Trm112 p the presence of Trm112p dramatically improves the methyltransferase activity of Trm9p in vitro 720827 2.1.1.229 Trm112p when produced in Escherichia coli, Trm112p forms a complex with Trm9p, which renders the latter soluble. This recombinant complex catalyzes the formation of mcm5U34 (5-methoxycarbonylmethyluridine) on tRNA in vitro but not mcm5s2U34 (5-methoxycarbonylmethyl-2-thiouridine) 712436