5.1.3.14 KCl 200 mM, twofold stimualtion 692855 5.1.3.14 additional information enzyme does not contain a tightly bound NAD+ 2281 5.1.3.14 additional information no activation by EDTA, and 1 mM DTT 747462 5.1.3.14 UDP-GlcNAc allosteric activator, absolutely required for their UDP-ManNAc 2-epimerization catalytic activity, activates recombinant His6-tagged enzyme NmSacA 747462 5.1.3.14 UDP-GlcNAc allosteric activitation by substrate via direct interactions between UDP and substrate 692076 5.1.3.14 UDP-N-acetyl-alpha-D-glucosamine activates the recombinant enzyme, UDP-GlcNAc is able to activate the epimerization process in a dose-dependent manner 747462 5.1.3.14 UDP-N-acetyl-D-glucosamine allosteric activation 661247 5.1.3.14 UDP-N-acetylglucosamine the reverse reaction with UDP-N-acetylmannosamine requires the presence of UDP-N-acetylglucosamine 2264, 2265, 2268