3.4.22.14 dithiothreitol activates 81474 3.4.22.14 L-cysteine for activation of actinidin, 52 mM L-cysteine is added to the sample and the preparation incubated for 1 h at 45°C 718203 3.4.22.14 L-cysteine the enzyme is activated in 20 mM potassium phosphate buffer, pH 6.5 containing 52 mM L-cysteine and 2 mM EDTA (1:1 (v/v)) for 1 h at 45°C 717622