2.7.3.3 acetate 5.5% activation at 50 mM 673167 2.7.3.3 ATP about 80% of the activity formerly inhibited by phenylglyoxal is retained by incubating with 20 mM ATP 674042 2.7.3.3 ATP after treatment with 10 mM ATP, the enzyme activity significantly increases 703776 2.7.3.3 dithiothreitol once treated with 5,5'-dithiobis-2-nitrobenzoic acid, the enzyme activity can be recovered more than 95% after incubation for 20 min with 0.15 mM dithiothreitol 674042 2.7.3.3 DMSO protects arginine kinase from inactivation losing its native tertiary conformation and aggregation in the presence of guanidine hydrochloride 674041 2.7.3.3 glycerol protects arginine kinase from inactivation in both low and high concentrations of guanidine hydrochloride 674041 2.7.3.3 hydrogen peroxide produces up to 10fold increase in enzyme expression at 0.2 mM 673489 2.7.3.3 additional information not affected by sodium nitroprusside, methylene blue and benznidazole 673489 2.7.3.3 nifurtimox produces 2.3fold increase in enzyme expression at 0.01 mM 673489 2.7.3.3 proline with proline the residual activity of AK after incubation in guanidine hydrochloride is higher than without proline 674041