1.14.15.8 heme the heme content of cytochrome P-450meg is 0.94 nmol of heme per nmol of cytochrome P-450 698682 1.14.15.8 Iron it is possible to resolve the hydroxylase system into three proteins: a strictly NADPH-dependent FMN-containing flavoprotein (megaredoxin reductase), an iron-sulfur protein (megaredoxin), and cytochrome P-450 (P-450meg). The activity of the 15beta-hydroxylase system is fully reconstituted upon combination of these three proteins and addition of NADPH. Megaredoxin has an apparent sulfur to iron ratio of 0.98 and shows g-signals at 1.90, 1.93, and 2.06 when analyzed by electron paramagnetic resonance spectroscopy 698682 1.14.15.8 phosphatidylcholine stimulates 698684