5.4.99.5 3,4-dimethoxycinnamic acid activates enzyme form CM3 3538, 3539 5.4.99.5 3,4-dimethoxycinnamic acid enzyme form CM1 is unaffected 3539 5.4.99.5 3,4-dimethoxycinnamic acid enzyme form CM2 is unaffected 3539 5.4.99.5 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase - 701494 5.4.99.5 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase the catalytic efficiency of chorismate mutase increases 140fold on addition of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, chorismate mutase forms a complex with 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and that complex formation both increases the CM activity by more than two orders of magnitude and endows chorismate mutase with regulatory features 703449 5.4.99.5 arabinose expression can be induced by arabinose under the control of the araBAD promoter 694070 5.4.99.5 caffeic acid enzyme form CM3 is activated 3539 5.4.99.5 cysteine - 747032, 748178 5.4.99.5 histidine - 747032, 748178 5.4.99.5 histidine is a positive effector for the enzyme 747032 5.4.99.5 L-Trp - 3554 5.4.99.5 L-Trp activates 3533, 3539, 3551 5.4.99.5 L-Trp activates enzyme forms CM1 and CM3 3538 5.4.99.5 L-Trp enzyme form CM1 is activated 3539, 3564 5.4.99.5 L-Trp enzyme form CM2 is unaffected 3539, 3551, 3564 5.4.99.5 L-Trp enzyme form CM3 is activated 3539 5.4.99.5 L-Trp no effect 3540, 3555 5.4.99.5 L-Trp plastidic isoenzyme is activated, cytosolic isoenzyme is inactive 3561 5.4.99.5 L-Trp wild-type enzyme, mutant enzyme Ile225Thr/Thr226Ile and enzymes with mutations at Tyr234 are activated. No activation of mutant enzyme Thr226Ile 3572 5.4.99.5 additional information although AtCM2 contains the putative regulatory effector binding domain, phenylalanine, tyrosine, and tryptophan do not affect its activity 748178 5.4.99.5 additional information CM1 is allosterically regulated by L-tryptophan but not L-phenylalanine or L-tyrosine 706241 5.4.99.5 additional information CM2 is not allosterically regulated by L-tryptophan, L-phenylalanine, or L-tyrosine 706241 5.4.99.5 additional information isozyme AtCM3 is unaltered by either phenylalanine or tyrosine but is activated by tryptophan, histidine, and cysteine 747032 5.4.99.5 additional information Most essential residue in BsCM is Arg90, the lack of Arg90 leads to a charge loss of catalytic activity. Two important catalytic roles of Arg90: one is to control the relative stability of the substrate through the collective hydrogen-bonding network in the Glu78-Arg90-substrate, and the other is to polarize the substrate at the appropriate location on the reaction path to gain the maximum electrostatic stabilisation factor for TSS 675611 5.4.99.5 additional information neither pH variation between 5.9 and 8.7, nor provision of 0.1 mg/ml bovine serum albumin, 2 mM Ca2+, 10 mM Mg2+, 1 mM EDTA, 1 mM EGTA, 1 mM 1,10-phenanthroline, 1 mM L-phenylalanine, 1 mM L-tyrosine, 1 mM L-tryptophan, or 0.6 mM salicylate affect catalytic activity by more than a factor of 2 703449 5.4.99.5 additional information neither tyrosine nor phenylalanine alters the activity of enzyme SmCM 747032 5.4.99.5 additional information the CM0819 activity is not affected by L-Phe, L-Tyr or L-Trp (0.01-10 mM), EDTA at concentrations of 0.1-2 mM does not affect CM0819 activity 705570 5.4.99.5 tryptophan - 747032, 748178 5.4.99.5 tryptophan 0.005 mM, heterotrophic activator 651936 5.4.99.5 tryptophan activates about 3fold 748178 5.4.99.5 tryptophan heterotrophic positive effector 651660 5.4.99.5 tryptophan is a positive effector for the enzyme 747032 5.4.99.5 tryptophan is a positive effector for the enzyme, identification of the allosteric effector site and the structural differences between the R- (more active) and T-state (less active) forms of plant chorismate mutase 747032