3.2.1.123	Activator protein	0.2 mg/ml	646698	
3.2.1.123	Activator protein	activator protein II is specific for EGCase II	646692, 646697	
3.2.1.123	Activator protein	increases the activity of EGCase II 468fold to 616fold	646693	
3.2.1.123	Activator protein	purified from culture supernatant of Rhodococcus sp. M-777, enhances the hydrolysis of glycosphingolipids in vitro and on the cell-surface by EGCase in absence of detergent	646690	
3.2.1.123	detergent	required	646690, 646692, 646695	
3.2.1.123	dimethylformamide	the activity is enhanced by addition of 5% (v/v) dimethylformamide	732295	
3.2.1.123	Dimethylsulfoxide	the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide	732295	
3.2.1.123	Lubrol Px	0.4% (w/v)	665547	
3.2.1.123	Lubrol Px	optimal concentration for C9 enzyme: 0.1%, no activation of M-777 enzyme	646702	
3.2.1.123	Lubrol Px	optimal concentration: 0.4%	646695	
3.2.1.123	additional information	for maximal hydrolysis of glycosphingolipids by rhodococcal EGCases, a detergent such as Triton X-100 is required at a final concentration of 0.1-0.3% of the reaction mixture. The hydrolysis of water soluble gangliosides such as GM1 does not require the presence of a detergent. For the hydrolysis of GM1, the addition of 5% tetrahydrofuran instead of Triton X-100 increases the reaction rate of EGCase I by 2fold compared to that in the absence of a detergent. Tetrahydrofuran is ineffective in facilitating the hydrolysis of GbOse4Cer by EGCase I. DMSO, ethyl acetate, and methyl cellosolve are also not effective in stimulating the hydrolysis of GM1 or GbOse4Cer by EGCase I in the absence of a detergent	698135	
3.2.1.123	Nonidet P-40	activates	646691	
3.2.1.123	Nonidet P-40	activates at 0.2%	646688	
3.2.1.123	sodium cholate	-	646691	
3.2.1.123	sodium taurodeoxycholate	activates endoglycoceramidase I and II, inhibition of endoglycoceramidase I above 0.4% w/v	646691	
3.2.1.123	taurodeoxycholate	0.5 mg/ml, 2.5fold increase of activity	646687	
3.2.1.123	tetrahydrofuran	the activity is enhanced by addition of 5% (v/v) tetrahydrofuran	732295	
3.2.1.123	Triton X-100	0.2% (w/v)	665547	
3.2.1.123	Triton X-100	activates	646691	
3.2.1.123	Triton X-100	activates at 0.2%	646688, 646695	
3.2.1.123	Triton X-100	activates endoglyceroceramidase I at 0.3-0.4%	646691	
3.2.1.123	Triton X-100	activates endoglyceroceramidase II at 0.2-0.4%	646691	
3.2.1.123	Triton X-100	optimum concentration for C9 and M-777 enzyme: 0.1-0.25%	646702	
3.2.1.123	Triton X-100	the activity is enhanced by addition of 0.1% (v/v) Triton X-100	732295