2.8.4.1	ADP	in absence of reducing agents	393255	
2.8.4.1	arsenate	in absence of reducing agents	393255	
2.8.4.1	ATP	-	393255	
2.8.4.1	ATP	absolute requirement, activation of the methylreductase system and not at the catalytic level	393257	
2.8.4.1	ATP	stimulates the reactivation	393251	
2.8.4.1	CN-B12	-	393257	
2.8.4.1	Cobalamin	-	393255	
2.8.4.1	Cobalamin	aquocobalamin	393256	
2.8.4.1	coenzyme F-420	enzyme system is stimulated	393257	
2.8.4.1	dithiothreitol	-	393255, 393256, 393257	
2.8.4.1	EDTA	in absence of reducing agents	393255	
2.8.4.1	H2	suggested to be involved in the activation of the methylreductase, preincubation	393257	
2.8.4.1	additional information	a method is reported to generate the active form of enzyme by adding sodium sulfide to a growing cell culture prior to harvest	726929	
2.8.4.1	additional information	activated enzyme immediately loses 97% of activity when assayed in the absence of Ti(III) citrate, dithiothreitol and cobalamin, that shows the requirement of these reductants for maximal activity	393255	
2.8.4.1	additional information	maximal stimulation with 0.3 mM aquocobalamin and 0.15 mM Ti(III) citrate	393256	
2.8.4.1	additional information	omission of the component A, B or C, as well as methyl coenzyme M, H2, Mg2+ or ATP leads to complete loss of activity and methane biosynthesis	393259	
2.8.4.1	phosphate	in absence of reducing agents	393255	
2.8.4.1	reduced corrinoids	-	393256	
2.8.4.1	reduced ferredoxin	reactivation after purification	393251	
2.8.4.1	sulfate	in absence of reducing agents	393255	
2.8.4.1	Ti(III) citrate	-	393255, 393256	
2.8.4.1	Ti(III) citrate	activation of the oxidized state of enzyme	393246	
2.8.4.1	Ti(III) citrate	artificial electron donor, if used as electron source component A1 is no longer required	393257	
2.8.4.1	Ti(III) citrate	conversion from the ox1 to the red1 state, maximal activation after 30 min, 20 mM Ti(III) at pH 9.0 and room temperature or pH 7.0 and 60°C, optimal pH for activation of isoenzyme II is 10.0, enzyme is activated when the enzyme bound coenzyme F-430 is reduced to the Ni(I) state	393247	
2.8.4.1	Ti(III) citrate	nickel tetrahydrocorphin	393258	
2.8.4.1	Ti(III) citrate	prevents enzyme inactivation by oxygen	393252	
2.8.4.1	Ti(III) citrate	reactivation after isolation	393251	
2.8.4.1	vitamin B12	enzyme system is stimulated	393257