2.7.1.100 2-mercaptoethanol 20 mM, 70% of activity with dithiothreitol 2.7.1.100 dithiothreitol required for activity, maximal activity at 20 mM 2.7.1.100 dithiothreitol can partially be replaced by 20 mM 2-mercaptoethanol or glutathione 2.7.1.100 glutathione 20 mM, 44% of activity with dithiothreitol 2.7.1.100 additional information strong upregulation of enzyme expression during sulfur starvation