2.4.2.30 DNA absolute requirement 637991, 637993 2.4.2.30 GDP increases activity in absence of Mg2+ 637994 2.4.2.30 GTP increases activity in absence of Mg2+ 637994 2.4.2.30 GTP(gammaS) increases activity in absence of Mg2+ 637994 2.4.2.30 ATP 5-10 mM, 20-30% stimulation 637995 2.4.2.30 4-[[[6-cyano-1-[(1-methyl-1H-imidazol-5-yl)methyl]-1,2,3,4,6,7-hexahydroquinolin-3-yl](pyridin-2-ylsulfonyl)amino]methyl]-N,N-dimethylpiperidine-1-carboxamide activates in presence of Mg2+, inhibits in absence of Mg2+ 637997 2.4.2.30 harmaline hydrochloride activates more strongly in absence of Mg2+ than in presence of Mg2+ 637997 2.4.2.30 Phthalic acid activates more strongly in absence of Mg2+ than in presence of Mg2+ 637997 2.4.2.30 DNA required 637998 2.4.2.30 DNA the enzyme is completely dependent on the presence of DNA containing single or double stranded breaks. Activation results in a decondensation of chromatin superstructure in vitro, which is caused mainly by hyper(ADP-ribosyl)ation of histone H1 637999 2.4.2.30 DNA enzyme has an N-terminal binding domain 638000 2.4.2.30 additional information in native conformation, CRM66 shows limited ability to modify EF-2 covalently. Upon activation with urea and dithiothreitol CRM66 loses ADP-ribosylation activity entirely, yet it retains the ability to bind NAD+. Replacement of Tyr-426 with histidine in CRM66 completely restores cytotoxicity and ADP-ribosyltransferase activity 662087 2.4.2.30 FAS exoenzyme S absolutely requires a soluble eukaryotic protein, named FAS (Factor Activating exoenzyme E), in order to ADP-ribosylate all substrates. In the presence of FAS, exoenzyme S ADP-ribosylates several proteins in lysates of Pseudomonas aeruginosa. Purification and characterization of FAS 662089 2.4.2.30 additional information PARP-1 is activated in response to DNA damage and participates in DNA repair, genomic integrity and cell death 671255 2.4.2.30 additional information stable expression of the transcription factor tonicity-responsive enhancer/osmotic response element-binding protein, TonEBP/OREBP, clone KIAA0827/amino acids 1-547, in HEK-293 cells increases the expression of the enzyme 671265 2.4.2.30 additional information DNA binding by PARP-1 triggers its activity and it adds poly(ADP-ribose) polymers to itself and to surrounding histones, overview 672969 2.4.2.30 protein 14-3-3 dependent on 672996 2.4.2.30 protein 14-3-3 required, interaction analysis, interaction equires residues L426, D427, and L428, overview, binding study of mutant enzymes, overview 673573 2.4.2.30 3-amino-1-methyl-5H-pyrido[4,3-b]indole i.e. Trp-P-2, 34% activation at 1 mM, 7% inhibition at 5 mM, IC50: 2.2 mM 675627 2.4.2.30 Bmh1p a yeast homologue of the human FAS, acts as an activating ExoS cofactor, overview 694800 2.4.2.30 Mg2+ 5 mM, 6.3fold activation 725034 2.4.2.30 Phenanthroline 0.1 mM, 1.1fold activation 725034 2.4.2.30 DNA slightly increases activity 725600 2.4.2.30 oligodeoxyribonucleotides slightly enhance enzyme activity with the maximal increase of 50% as compared to the control 725600 2.4.2.30 peptide 14-3-3beta - 736518