1.13.11.27 1-butanol 5% v/v, 2.9fold activation 395377 1.13.11.27 1-propanol 10% v/v, 2.1fold activation 395377 1.13.11.27 2,6-dichlorophenol indophenol competitive activator 395384 1.13.11.27 2,6-dichlorophenol indophenol reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione 395368 1.13.11.27 2,6-dichlorophenol indophenol stimulates 395390 1.13.11.27 acetone 10% v/v, 5.7fold activation 395377 1.13.11.27 acetonitrile 10% v/v, 3.6fold activation 395377 1.13.11.27 ascorbate an optimized combination of ascorbate, 50 mM, catalase, 2.4 g/l, and iron, 0.05 mM, activates more than a combination of 2,6-dichlorophenolindophenol, glutathione and catalase 395384 1.13.11.27 ascorbate reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase 395368, 395381 1.13.11.27 ascorbate required for activity 658081 1.13.11.27 catalase from bovine liver, required for activity 658081 1.13.11.27 Cyclohexanol 5% v/v, 4.4fold activation 395377 1.13.11.27 dioxane 10% v/v, 2.8fold activation 395377 1.13.11.27 ethanol 10% v/v, 2fold activation 395377 1.13.11.27 ether 10% v/v, 2.6fold activation 395377 1.13.11.27 glutathione reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase 395368, 395381 1.13.11.27 methanol 10% v/v, 1.6fold activation 395377 1.13.11.27 methyl-cellosolve 10% v/v, 2.2fold activation 395377 1.13.11.27 additional information regarding expression and acitvity, rich or complex media (surplus of yeast extract, addition of glycerol and buffering of pH) are superior to media with less complex composition. Supplementation of media with FeSO4 or tyrosine does not substantially upgrade expression and activity levels. 677278 1.13.11.27 tetrahydrofuran 10% v/v, 6.7fold activation 395377 1.13.11.27 Trypsin liver enzyme appears to be present in an inactive form which can be activated by treatment with trypsin or by autolysis 395388