1.13.11.20 2,2'-dipyridyl slight activation at 0.01 mM 439546 1.13.11.20 8-hydroxyquinoline slight activation at 0.01 mM 439546 1.13.11.20 Carboxyethyl-L-cysteine at 10 mM, activation 439542 1.13.11.20 carboxymethyl-L-cysteine at 10 mM, activation 439542 1.13.11.20 cysteamine - 439548 1.13.11.20 cysteamine at 100 mM, activation 439542 1.13.11.20 cysteamine at a reaction time of 45 min, CDO activity increases almost 20times in the presence of 5.8 mM cysteamine 674569 1.13.11.20 cysteine protein expression of recombinant wild-type enzyme in HepG2/C3A cells increases by 160% when extracellular cysteine levels are increased from 0 to 1 mM cysteine 684337 1.13.11.20 D-cysteine at 10 mM, activation 439542 1.13.11.20 diethyldithiocarbamate without protein-A: 30% activation at 0.1 mM 439548 1.13.11.20 DL-homocysteine - 439548 1.13.11.20 DL-homocysteine at 100 mM, slight activation 439542 1.13.11.20 DL-homocysteine increases activity 439554 1.13.11.20 ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity 741704 1.13.11.20 Fe2+ stimulates 439552 1.13.11.20 hydrocortisone induces 439542 1.13.11.20 hydroxylamine activates, restores the inhibition by Fe2+ 439547 1.13.11.20 L-cysteine activates the purified enzyme under anaerobic conditions 439546, 439548 1.13.11.20 L-cysteine acts as an initial signal for regulation of the enzyme, upregulates enzyme activity 439554 1.13.11.20 L-cysteine induces the enzyme 439553, 439554 1.13.11.20 L-cysteine upregulates the enzyme in hepatic and brain cells 439557 1.13.11.20 methionine increases enzyme activity 439554 1.13.11.20 methionine induces 439542, 439553, 439554 1.13.11.20 additional information covalent post-translational modification between the residues C93 and Y157, in close proximity to the active site, enhances the enzyme's activity. The presence of ferrous iron and oxygen is a prerequisite for C93-Y157 crosslink formation. Both the enzymatic rate of cysteine oxidation and the amount of cross-linking between C93 and Y157 increased significantly upon exposure of CDO to air/oxygen and substrate cysteine in the presence of iron in a hitherto unreported two-phase process. The non-crosslinked form has negligible enzymatic activity 724430 1.13.11.20 additional information cysteine mediates the up-regulation of enzyme in cultured hepatocytes 659767 1.13.11.20 additional information effect of dietary protein or cystine on CDO expression and enzyme activity in rat liver and adipose tissue 693792 1.13.11.20 additional information the enzyme activity is in part modulated by the formation of a Cys93-Tyr157 crosslink that increases its catalytic efficiency over 10fold, mechanism, overview. The crosslink enhances activity by positioning the Tyr157 hydroxyl to enable proper Cys binding, proper oxygen binding, and optimal chemistry 743088 1.13.11.20 additional information with pure cysteine dioxygenase no effect of addition of NAD+ to the cysteine diosygenase is found. 676962 1.13.11.20 N-acetyl-L-cysteine - 439548 1.13.11.20 N-acetyl-L-cysteine at 100 mM, slight activation 439542 1.13.11.20 NAD(P)H required for fully activity 439544 1.13.11.20 NAD(P)H stimulation 439542 1.13.11.20 NAD+ stimulates 439552 1.13.11.20 NADH enzyme uses NAD+/NADH as pharmacological chaperone. Presence of 0.1 mM NADH increase activity 1.3fold 765261 1.13.11.20 NADH stimulates 439543 1.13.11.20 Neocuproine without protein-A: slight activation at 0.1 mM 439548 1.13.11.20 S-methyl-L-cysteine - 439548 1.13.11.20 S-methyl-L-cysteine at 10 mM, activation 439542