2.4.2.30	GDP	increases activity in absence of Mg2+	637994	
2.4.2.30	GTP	increases activity in absence of Mg2+	637994	
2.4.2.30	GTP(gammaS)	increases activity in absence of Mg2+	637994	
2.4.2.30	harmaline hydrochloride	activates more strongly in absence of Mg2+ than in presence of Mg2+	637997	
2.4.2.30	Mg2+	5 mM, 6.3fold activation	725034	
2.4.2.30	additional information	DNA binding by PARP-1 triggers its activity and it adds poly(ADP-ribose) polymers to itself and to surrounding histones, overview	672969	
2.4.2.30	additional information	in native conformation, CRM66 shows limited ability to modify EF-2 covalently. Upon activation with urea and dithiothreitol CRM66 loses ADP-ribosylation activity entirely, yet it retains the ability to bind NAD+. Replacement of Tyr-426 with histidine in CRM66 completely restores cytotoxicity and ADP-ribosyltransferase activity	662087	
2.4.2.30	additional information	PARP-1 is activated in response to DNA damage and participates in DNA repair, genomic integrity and cell death	671255	
2.4.2.30	additional information	stable expression of the transcription factor tonicity-responsive enhancer/osmotic response element-binding protein, TonEBP/OREBP, clone KIAA0827/amino acids 1-547, in HEK-293 cells increases the expression of the enzyme	671265	
2.4.2.30	oligodeoxyribonucleotides	slightly enhance enzyme activity with the maximal increase of 50% as compared to the control	725600