BRENDA - Enzyme Database

Some properties of pantothenate synthetase partially purified from Escherichia coli B

Miyatake, K.; Nakano, Y.; Kitaoka, S.; Bull. Univ. Osaka Prefect 27, 57-67 (1975)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.1
1,10-phenanthroline
-
Escherichia coli
6.3.2.1
4-aminobutanoate
-
Escherichia coli
6.3.2.1
5-Aminopentanoate
-
Escherichia coli
6.3.2.1
Ag+
-
Escherichia coli
6.3.2.1
AMP
-
Escherichia coli
6.3.2.1
Asp
uncompetitive
Escherichia coli
6.3.2.1
Ca2+
-
Escherichia coli
6.3.2.1
Cu2+
-
Escherichia coli
6.3.2.1
cysteine
weak
Escherichia coli
6.3.2.1
diphosphate
-
Escherichia coli
6.3.2.1
DTT
weak
Escherichia coli
6.3.2.1
Fe2+
-
Escherichia coli
6.3.2.1
Hg2+
-
Escherichia coli
6.3.2.1
iodoacetate
weak
Escherichia coli
6.3.2.1
L-Ala
-
Escherichia coli
6.3.2.1
L-Val
uncompetitive
Escherichia coli
6.3.2.1
Li+
-
Escherichia coli
6.3.2.1
mercaptoethanol
weak
Escherichia coli
6.3.2.1
Mercaptoethanolamine
weak
Escherichia coli
6.3.2.1
Mercaptopurine
weak
Escherichia coli
6.3.2.1
Ni2+
-
Escherichia coli
6.3.2.1
pantothenic acid
-
Escherichia coli
6.3.2.1
Pb2+
-
Escherichia coli
6.3.2.1
PCMB
-
Escherichia coli
6.3.2.1
taurine
-
Escherichia coli
6.3.2.1
Zn2+
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.1
0.063
-
D-pantoate
beta-alanine
Escherichia coli
6.3.2.1
0.091
-
ATP
-
Escherichia coli
6.3.2.1
0.12
-
Pantoate
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.1
K+
Km: 5.5 mM
Escherichia coli
6.3.2.1
Mg2+
divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.1
69000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.2.1
ATP + (R)-pantoate + beta-alanine
Escherichia coli
formation of pantothenic acid, an important member of the B vitamins
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.2.1
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.2.1
partial
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.1
ATP + (R)-pantoate + beta-alanine
-
979
Escherichia coli
AMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
ATP + (R)-pantoate + beta-alanine
formation of pantothenic acid, an important member of the B vitamins
979
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.2.1
30
-
-
Escherichia coli
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.3.2.1
20
50
20°C: about 60% of maximal activity, 50°C, about 25% of maximal activity
Escherichia coli
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.2.1
37
-
pH 5.0-10.0, 15 min stable
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.1
10
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.3.2.1
8
12
about 50% of maximal activity at pH 8 and 12
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.1
1,10-phenanthroline
-
Escherichia coli
6.3.2.1
4-aminobutanoate
-
Escherichia coli
6.3.2.1
5-Aminopentanoate
-
Escherichia coli
6.3.2.1
Ag+
-
Escherichia coli
6.3.2.1
AMP
-
Escherichia coli
6.3.2.1
Asp
uncompetitive
Escherichia coli
6.3.2.1
Ca2+
-
Escherichia coli
6.3.2.1
Cu2+
-
Escherichia coli
6.3.2.1
cysteine
weak
Escherichia coli
6.3.2.1
diphosphate
-
Escherichia coli
6.3.2.1
DTT
weak
Escherichia coli
6.3.2.1
Fe2+
-
Escherichia coli
6.3.2.1
Hg2+
-
Escherichia coli
6.3.2.1
iodoacetate
weak
Escherichia coli
6.3.2.1
L-Ala
-
Escherichia coli
6.3.2.1
L-Val
uncompetitive
Escherichia coli
6.3.2.1
Li+
-
Escherichia coli
6.3.2.1
mercaptoethanol
weak
Escherichia coli
6.3.2.1
Mercaptoethanolamine
weak
Escherichia coli
6.3.2.1
Mercaptopurine
weak
Escherichia coli
6.3.2.1
Ni2+
-
Escherichia coli
6.3.2.1
pantothenic acid
-
Escherichia coli
6.3.2.1
Pb2+
-
Escherichia coli
6.3.2.1
PCMB
-
Escherichia coli
6.3.2.1
taurine
-
Escherichia coli
6.3.2.1
Zn2+
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.1
0.063
-
D-pantoate
beta-alanine
Escherichia coli
6.3.2.1
0.091
-
ATP
-
Escherichia coli
6.3.2.1
0.12
-
Pantoate
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.1
K+
Km: 5.5 mM
Escherichia coli
6.3.2.1
Mg2+
divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.1
69000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.2.1
ATP + (R)-pantoate + beta-alanine
Escherichia coli
formation of pantothenic acid, an important member of the B vitamins
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.1
partial
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.1
ATP + (R)-pantoate + beta-alanine
-
979
Escherichia coli
AMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
ATP + (R)-pantoate + beta-alanine
formation of pantothenic acid, an important member of the B vitamins
979
Escherichia coli
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.2.1
30
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.3.2.1
20
50
20°C: about 60% of maximal activity, 50°C, about 25% of maximal activity
Escherichia coli
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.2.1
37
-
pH 5.0-10.0, 15 min stable
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.1
10
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.3.2.1
8
12
about 50% of maximal activity at pH 8 and 12
Escherichia coli