BRENDA - Enzyme Database show

Enzymological properties of pantothenate synthetase from Escherichia coli B

Miyatake, K.; Nakano, Y.; Kitaoka, S.; J. Nutr. Sci. Vitaminol. 24, 243-253 (1978)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.3.2.1
lyophilized enzyme is very stable
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.1
1,10-phenanthroline
-
Escherichia coli
6.3.2.1
2,3-Diaminopropanoate
slight
Escherichia coli
6.3.2.1
2-hydroxybutanoate
slight
Escherichia coli
6.3.2.1
2-Hydroxypropanoate
slight
Escherichia coli
6.3.2.1
3-hydroxybutanoate
slight
Escherichia coli
6.3.2.1
3-hydroxypropanoate
slight
Escherichia coli
6.3.2.1
4-Amino-3-hydroxybutanoate
-
Escherichia coli
6.3.2.1
4-aminobutanoate
-
Escherichia coli
6.3.2.1
5-Aminopentanoate
slight
Escherichia coli
6.3.2.1
6-aminohexanoate
slight
Escherichia coli
6.3.2.1
Ag+
-
Escherichia coli
6.3.2.1
anserine
slight
Escherichia coli
6.3.2.1
Ba2+
-
Escherichia coli
6.3.2.1
Ca2+
-
Escherichia coli
6.3.2.1
carnosine
slight
Escherichia coli
6.3.2.1
Co2+
-
Escherichia coli
6.3.2.1
Cu2+
-
Escherichia coli
6.3.2.1
EDTA
-
Escherichia coli
6.3.2.1
EGTA
-
Escherichia coli
6.3.2.1
Fe2+
-
Escherichia coli
6.3.2.1
gluconate
32% inhibition at 1 mM
Escherichia coli
6.3.2.1
glycolate
30% inhibition at 1 mM
Escherichia coli
6.3.2.1
Hg2+
-
Escherichia coli
6.3.2.1
iodoacetate
-
Escherichia coli
6.3.2.1
L-Ala
-
Escherichia coli
6.3.2.1
L-cysteate
-
Escherichia coli
6.3.2.1
L-Val
-
Escherichia coli
6.3.2.1
Lactate
-
Escherichia coli
6.3.2.1
Ni2+
slight
Escherichia coli
6.3.2.1
Sn2+
slight
Escherichia coli
6.3.2.1
Sr2+
slight
Escherichia coli
6.3.2.1
taurine
-
Escherichia coli
6.3.2.1
Zn2+
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.1
0.063
-
D-pantoate
-
Escherichia coli
6.3.2.1
0.1
-
ATP
-
Escherichia coli
6.3.2.1
0.15
-
beta-Alanine
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.1
K+
combination of divalent and monovalent cation required
Escherichia coli
6.3.2.1
Mg2+
divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above
Escherichia coli
6.3.2.1
Mn2+
combination of divalent and monovalent cation required; divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above
Escherichia coli
6.3.2.1
NH4+
combination of divalent and monovalent cation required
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.2.1
Escherichia coli
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
6.3.2.1
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
bi uni uni bi ping pong mechanism
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
6.3.2.1
-20°C, stable for 6 months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.1
ATP + (R)-pantoate + beta-alanine
-
978
Escherichia coli
AMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
CTP + (R)-pantoate + beta-alanine
14% of the activity relative to ATP
978
Escherichia coli
CMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
GTP + (R)-pantoate + beta-alanine
12% of the activity relative to ATP
978
Escherichia coli
GMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
ITP + (R)-pantoate + beta-alanine
14% of the activity relative to ATP
978
Escherichia coli
IMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
UTP + (R)-pantoate + beta-alanine
16% of the activity relative to ATP
978
Escherichia coli
UMP + diphosphate + (R)-pantothenate
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.2.1
30
-
-
Escherichia coli
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.2.1
60
-
10 min, complete loss of activity
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.1
10
-
-
Escherichia coli
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
6.3.2.1
5
11
15 min, 40°C, stable
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
6.3.2.1
lyophilized enzyme is very stable
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.1
1,10-phenanthroline
-
Escherichia coli
6.3.2.1
2,3-Diaminopropanoate
slight
Escherichia coli
6.3.2.1
2-hydroxybutanoate
slight
Escherichia coli
6.3.2.1
2-Hydroxypropanoate
slight
Escherichia coli
6.3.2.1
3-hydroxybutanoate
slight
Escherichia coli
6.3.2.1
3-hydroxypropanoate
slight
Escherichia coli
6.3.2.1
4-Amino-3-hydroxybutanoate
-
Escherichia coli
6.3.2.1
4-aminobutanoate
-
Escherichia coli
6.3.2.1
5-Aminopentanoate
slight
Escherichia coli
6.3.2.1
6-aminohexanoate
slight
Escherichia coli
6.3.2.1
Ag+
-
Escherichia coli
6.3.2.1
anserine
slight
Escherichia coli
6.3.2.1
Ba2+
-
Escherichia coli
6.3.2.1
Ca2+
-
Escherichia coli
6.3.2.1
carnosine
slight
Escherichia coli
6.3.2.1
Co2+
-
Escherichia coli
6.3.2.1
Cu2+
-
Escherichia coli
6.3.2.1
EDTA
-
Escherichia coli
6.3.2.1
EGTA
-
Escherichia coli
6.3.2.1
Fe2+
-
Escherichia coli
6.3.2.1
gluconate
32% inhibition at 1 mM
Escherichia coli
6.3.2.1
glycolate
30% inhibition at 1 mM
Escherichia coli
6.3.2.1
Hg2+
-
Escherichia coli
6.3.2.1
iodoacetate
-
Escherichia coli
6.3.2.1
L-Ala
-
Escherichia coli
6.3.2.1
L-cysteate
-
Escherichia coli
6.3.2.1
L-Val
-
Escherichia coli
6.3.2.1
Lactate
-
Escherichia coli
6.3.2.1
Ni2+
slight
Escherichia coli
6.3.2.1
Sn2+
slight
Escherichia coli
6.3.2.1
Sr2+
slight
Escherichia coli
6.3.2.1
taurine
-
Escherichia coli
6.3.2.1
Zn2+
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.1
0.063
-
D-pantoate
-
Escherichia coli
6.3.2.1
0.1
-
ATP
-
Escherichia coli
6.3.2.1
0.15
-
beta-Alanine
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.1
K+
combination of divalent and monovalent cation required
Escherichia coli
6.3.2.1
Mg2+
divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above
Escherichia coli
6.3.2.1
Mn2+
combination of divalent and monovalent cation required; divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above
Escherichia coli
6.3.2.1
NH4+
combination of divalent and monovalent cation required
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.3.2.1
-20°C, stable for 6 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.1
ATP + (R)-pantoate + beta-alanine
-
978
Escherichia coli
AMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
CTP + (R)-pantoate + beta-alanine
14% of the activity relative to ATP
978
Escherichia coli
CMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
GTP + (R)-pantoate + beta-alanine
12% of the activity relative to ATP
978
Escherichia coli
GMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
ITP + (R)-pantoate + beta-alanine
14% of the activity relative to ATP
978
Escherichia coli
IMP + diphosphate + (R)-pantothenate
-
-
-
-
6.3.2.1
UTP + (R)-pantoate + beta-alanine
16% of the activity relative to ATP
978
Escherichia coli
UMP + diphosphate + (R)-pantothenate
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.2.1
30
-
-
Escherichia coli
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.2.1
60
-
10 min, complete loss of activity
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.1
10
-
-
Escherichia coli
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
6.3.2.1
5
11
15 min, 40°C, stable
Escherichia coli