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Literature summary extracted from

  • Miyatake, K.; Nakano, Y.; Kitaoka, S.
    Enzymological properties of pantothenate synthetase from Escherichia coli B (1978), J. Nutr. Sci. Vitaminol., 24, 243-253.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.1 lyophilized enzyme is very stable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.1 1,10-phenanthroline
-
 Escherichia coli
6.3.2.1 2,3-Diaminopropanoate slight Escherichia coli
6.3.2.1 2-hydroxybutanoate slight Escherichia coli
6.3.2.1 2-Hydroxypropanoate slight Escherichia coli
6.3.2.1 3-hydroxybutanoate slight Escherichia coli
6.3.2.1 3-hydroxypropanoate slight Escherichia coli
6.3.2.1 4-Amino-3-hydroxybutanoate
-
 Escherichia coli
6.3.2.1 4-aminobutanoate
-
 Escherichia coli
6.3.2.1 5-Aminopentanoate slight Escherichia coli
6.3.2.1 6-aminohexanoate slight Escherichia coli
6.3.2.1 Ag+
-
 Escherichia coli
6.3.2.1 anserine slight Escherichia coli
6.3.2.1 Ba2+
-
 Escherichia coli
6.3.2.1 Ca2+
-
 Escherichia coli
6.3.2.1 carnosine slight Escherichia coli
6.3.2.1 Co2+
-
 Escherichia coli
6.3.2.1 Cu2+
-
 Escherichia coli
6.3.2.1 EDTA
-
 Escherichia coli
6.3.2.1 EGTA
-
 Escherichia coli
6.3.2.1 Fe2+
-
 Escherichia coli
6.3.2.1 gluconate 32% inhibition at 1 mM Escherichia coli
6.3.2.1 glycolate 30% inhibition at 1 mM Escherichia coli
6.3.2.1 Hg2+
-
 Escherichia coli
6.3.2.1 iodoacetate
-
 Escherichia coli
6.3.2.1 L-Ala
-
 Escherichia coli
6.3.2.1 L-cysteate
-
 Escherichia coli
6.3.2.1 L-Val
-
 Escherichia coli
6.3.2.1 Lactate
-
 Escherichia coli
6.3.2.1 Ni2+ slight Escherichia coli
6.3.2.1 Sn2+ slight Escherichia coli
6.3.2.1 Sr2+ slight Escherichia coli
6.3.2.1 taurine
-
 Escherichia coli
6.3.2.1 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.1 0.063
-
 D-pantoate
-
 Escherichia coli
6.3.2.1 0.1
-
 ATP
-
 Escherichia coli
6.3.2.1 0.15
-
 beta-Alanine
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.1 K+ combination of divalent and monovalent cation required Escherichia coli
6.3.2.1 Mg2+ divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above Escherichia coli
6.3.2.1 Mn2+ combination of divalent and monovalent cation required Escherichia coli
6.3.2.1 Mn2+ divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above Escherichia coli
6.3.2.1 NH4+ combination of divalent and monovalent cation required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.1 ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate bi uni uni bi ping pong mechanism Escherichia coli
a

Storage Stability

EC Number Storage Stability Organism
6.3.2.1 -20°C, stable for 6 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine
-
 Escherichia coli AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 CTP + (R)-pantoate + beta-alanine 14% of the activity relative to ATP Escherichia coli CMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 GTP + (R)-pantoate + beta-alanine 12% of the activity relative to ATP Escherichia coli GMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ITP + (R)-pantoate + beta-alanine 14% of the activity relative to ATP Escherichia coli IMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 UTP + (R)-pantoate + beta-alanine 16% of the activity relative to ATP Escherichia coli UMP + diphosphate + (R)-pantothenate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.1 30
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.1 60
-
 10 min, complete loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.1 10
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.1 5 11 15 min, 40°C, stable Escherichia coli