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Literature summary extracted from

  • Henderson, G.B.; Yamaguchi, M.; Novoa, L.; Fairlamb, A.H.; Cerami, A.
    Biosynthesis of the trypanosomatid metabolite trypanothione: purification and characterization of trypanothione synthetase from Crithidia fasciculata (1990), Biochemistry, 29, 3924-3929.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.9 0.007
-
 N8-glutathionylspermidine
-
 Crithidia fasciculata
6.3.1.9 0.02
-
 N1-glutathionylspermidine
-
 Crithidia fasciculata
6.3.1.9 0.4
-
 MgATP2-
-
 Crithidia fasciculata
6.3.1.9 0.914
-
 gamma-L-Glu-L-Cys-Gly
-
 Crithidia fasciculata
6.3.1.9 1.07
-
 spermidine
-
 Crithidia fasciculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.9 Mg2+ MgATP2- required Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.9 81000
-
 gel filtration Crithidia fasciculata
6.3.1.9 87000
-
 1 * 87000, SDS-PAGE Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.9 Crithidia fasciculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.9
-
 Crithidia fasciculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.9 additional information
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.9 gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O
-
 Crithidia fasciculata N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3 N1-glutathionylspermidine and N8-glutathionylspermidine are intermediates ?
6.3.1.9 glutathione + spermidine + ATP ATP in form of MgATP2- Crithidia fasciculata glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 N8-glutathionylspermidine + glutathione + ATP
-
 Crithidia fasciculata N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.9 monomer 1 * 87000, SDS-PAGE Crithidia fasciculata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.9 10
-
 N1-glutathionylspermidine
-
 Crithidia fasciculata
6.3.1.9 24.3
-
 N8-glutathionylspermidine
-
 Crithidia fasciculata
6.3.1.9 28.6
-
 spermidine
-
 Crithidia fasciculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.9 7.5 7.8 synthesis of both mono-glutathionylspermidine and di-glutathionylspermidine conjugates Crithidia fasciculata