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Literature summary extracted from

  • Viswanathan, S.; Dignam, J.D.
    Seryl-tRNA synthetase from Bombyx mori. Purification and properties (1988), J. Biol. Chem., 263, 535-541.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.11 glycerol and DTT enhance stability of the enzyme Bombyx mori
6.1.1.11 not particularly susceptible to inactivation from by freezing and thawing Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 0.00025
-
 tRNASer substrate from Bombyx mori Bombyx mori
6.1.1.11 0.00026
-
 tRNASer substrate from rabbit liver Bombyx mori
6.1.1.11 0.11
-
 ATP
-
 Bombyx mori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.11 Mg2+ required Bombyx mori
6.1.1.11 Mg2+ optimum concentration: 6 mM Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.11 60000
-
 2 * 60000, SDS-PAGE Bombyx mori
6.1.1.11 124000
-
 gel filtration, sedimentation velocity Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 additional information Bombyx mori high abundance in middle silk gland may result from an adaption of this organ for the production of the serine-rich protein, sericin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11
-
 Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.11 silk gland middle, high abundance in this organ may result from an adaption of this organ to the production of the serine-rich protein, sericin Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.11 10.87
-
-
 Bombyx mori

Storage Stability

EC Number Storage Stability Organism
6.1.1.11 4°C, 50 mM potassium phosphate buffer, pH 7.5, 20% glycerol, 5 mM magnesium acetate, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 1 week, less than 20% loss of activity Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Bombyx mori AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information high abundance in middle silk gland may result from an adaption of this organ for the production of the serine-rich protein, sericin Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.11 dimer 2 * 60000, SDS-PAGE Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.11 7.8
-
 Hepes or Tris-HCl buffer Bombyx mori