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Literature summary extracted from

  • Bollinger, J.M.; Kwon, D.S.; Huisman, G.W.; Kolter, R.; Walsh, C.T.
    Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase (1995), J. Biol. Chem., 270, 14031-14041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.78
-
 Escherichia coli
6.3.1.8
-
 Escherichia coli

General Stability

EC Number General Stability Organism
3.5.1.78 the presence of Mg2+, ATP and reduced glutathione, with or without spermidine, markedly protects the enzyme from cleavage by trypsin Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.78 0.9
-
 glutathionylspermidine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.78 70000
-
 or trimer, 2 or 3 * 70000, SDS-PAGE Escherichia coli
3.5.1.78 70000
-
 or dimer, 3 or 2 * 70000, SDS-PAGE Escherichia coli
3.5.1.78 170000
-
 gel filtration, the deviation of the estimated molecular mass from 140000 of a dimer might result from the dimer having a non-globular shape Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.78 Escherichia coli
-
 bifunctional enzyme glutathionylspermidine synthetase/amidase
-
6.3.1.8 Escherichia coli
-
 the two activities of the bifunctional enzyme reside in distinct domains
-
6.3.1.8 Escherichia coli
-
 the enzyme is bifunctional and also catalyzes the glutathionylspermidine amidase reaction, EC 3.5.1.78, resulting in a net hydrolysis of ATP
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.8
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.78 additional information
-
-
 Escherichia coli
6.3.1.8 7.4
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.78 glutathionylspermidine + H2O
-
 Escherichia coli glutathione + spermidine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.78 dimer or trimer, 2 or 3 * 70000, SDS-PAGE Escherichia coli
3.5.1.78 trimer or dimer, 3 or 2 * 70000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.78 2.1
-
 glutathionylspermidine
-
 Escherichia coli