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Literature summary extracted from

  • De Craecker, S.; Verbruggen, C.; Rajan, P.; Smith, K.; Haemers, A.; Fairlamb, A.H.
    Characterization of the peptide substrate specificity of glutathionylspermidine synthetase from Crithidia fasciculata (1997), Mol. Biochem. Parasitol., 84, 25-32.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.8 gamma-Aminobutyryl-L-Ala-Gly 5 mM, 10% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-D-Phe-Gly 5 mM, 10% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-Leu 5 mM, 10% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-Leu-Gly 5 mM, 58% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-leu-L-Ala 5 mM, 95% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-Phe-Gly 5 mM, 73% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-Ser-Gly 5 mM, 50% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-Val 5 mM, 10% inhibition Crithidia fasciculata
6.3.1.8 gamma-L-Glu-L-Val-L-Ala 5 mM, 58% inhibition Crithidia fasciculata
6.3.1.8 Glutaryl-L-Val-Gly 5 mM, 10% inhibition Crithidia fasciculata
6.3.1.8 L-beta-Asp-L-Leu-Gly 5 mM, 42% inhibition Crithidia fasciculata
6.3.1.8 L-beta-Asp-L-Val-Gly 5 mM, 15% inhibition Crithidia fasciculata
6.3.1.8 L-Cys-Gly 5 mM, 20% inhibition Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.8 0.13
-
 gamma-L-Glu-L-Cys(S-methyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 0.18
-
 gamma-Glu-L-Cys(S-ethyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 0.4
-
 gamma-L-Glu-L-Ile-Gly
-
 Crithidia fasciculata
6.3.1.8 0.53
-
 gamma-Glu-L-Cys(S-propyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 0.67
-
 gamma-L-Glu-L-Val-Gly
-
 Crithidia fasciculata
6.3.1.8 0.91
-
 gamma-L-Glu-L-Cys-Gly
-
 Crithidia fasciculata
6.3.1.8 4
-
 gamma-L-Glu-L-Ala-Gly
-
 Crithidia fasciculata
6.3.1.8 14
-
 gamma-Glu-L-Cys(S-butyl)-Gly
-
 Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Crithidia fasciculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.8
-
 Crithidia fasciculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.8 additional information
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.8 gamma-Glu-Gly-Gly + spermidine + ATP at 14% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-Glu-Gly-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-Glu-L-Cys(S-butyl)-Gly + spermidine + ATP at 24% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-Glu-L-Cys(S-butyl)-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-Glu-L-Cys(S-ethyl)-Gly + spermidine + ATP at 77% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-Glu-L-Cys(S-ethyl)-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-Glu-L-Cys(S-propyl)-Gly + spermidine + ATP at 56% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-Glu-L-Cys(S-propyl)-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-Glu-L-Ser-Gly + spermidine + ATP at 16% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-Glu-L-Ser-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glu-L-Ala-Gly + spermidine + ATP at 28% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-L-Glu-L-Ala-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glu-L-alpha-aminobutyryl-Gly + spermidine + ATP gamma-L-Glu-L-alpha-aminobutyryl-Gly i.e. ophthalmic acid Crithidia fasciculata N1-(gamma-L-Glu-L-alpha-aminobutyryl-Gly)-spermidine
-
 ?
6.3.1.8 gamma-L-Glu-L-Cys(S-methyl)-Gly + spermidine + ATP at 74% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-L-Glu-L-Cys(S-methyl)-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glu-L-Cys-Gly + spermidine + ATP
-
 Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glu-L-Ile-Gly + spermidine + ATP at 70% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-L-Glu-L-Ile-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glu-L-Val-Gly + spermidine + ATP at 88% of the activity with gamma-L-Glu-L-Cys-Gly Crithidia fasciculata N1-(gamma-L-Glu-L-Val-Gly)-spermidine + ADP + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.8 2.5
-
 gamma-L-Glu-L-Ala-Gly
-
 Crithidia fasciculata
6.3.1.8 8.7
-
 gamma-Glu-L-Cys(S-propyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 8.8
-
 gamma-L-Glu-L-Cys(S-methyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 9.2
-
 gamma-Glu-L-Cys(S-butyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 10
-
 gamma-L-Glu-L-Ile-Gly
-
 Crithidia fasciculata
6.3.1.8 11.3
-
 gamma-Glu-L-Cys(S-ethyl)-Gly
-
 Crithidia fasciculata
6.3.1.8 11.8
-
 gamma-L-Glu-L-Val-Gly
-
 Crithidia fasciculata
6.3.1.8 15
-
 gamma-L-Glu-L-Cys-Gly
-
 Crithidia fasciculata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.1.8 5
-
 gamma-L-Glu-L-Ser-Gly
-
 Crithidia fasciculata