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Literature summary extracted from

  • Tetaud, E.; Manai, F.; Barrett, M.P.; Nadeau, K.; Walsh, C.T.; Fairlamb, A.H.
    Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata (1998), J. Biol. Chem., 273, 19383-19390.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.8 expression in Escherichia coli and Saccharomyces cerevisiae Crithidia fasciculata
6.3.1.8 overexpression in Escherichia coli and Saccharomyces cerevisiae of the gene Cf-GSS yields insoluble proteins Crithidia fasciculata
6.3.1.9 expression in Escherichia coli and Saccharomyces cerevisiae Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.8 80340
-
 predicted from sequence Crithidia fasciculata
6.3.1.8 80640
-
 MALDI-TOF MS Crithidia fasciculata
6.3.1.8 90000
-
 1 * 90000, SDS-PAGE Crithidia fasciculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.8 gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP Crithidia fasciculata synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants ?
-
 ?
6.3.1.9 gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP Crithidia fasciculata synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Crithidia fasciculata
-
-
-
6.3.1.8 Crithidia fasciculata P90518
-
-
6.3.1.9 Crithidia fasciculata O60993
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.8 additional information
-
 enzyme cloned in Escherichia coli and Saccharomyces cerevisiae is inactive Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.8 gamma-L-Glu-L-Cys-Gly + spermidine + ATP
-
 Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2-
-
 Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate
-
 ?
6.3.1.8 gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants Crithidia fasciculata ?
-
 ?
6.3.1.9 gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants Crithidia fasciculata N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.8 monomer 1 * 90000, SDS-PAGE Crithidia fasciculata