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Literature summary extracted from

  • Smith, K.; Nadeau, K.; Bradley, M.; Walsh, C.; Fairlamb, A.H.
    Purification of glutathionylspermidine and trypanothione synthetase from Crithidia fasciculata (1992), Protein Sci., 1, 874-883.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.8 reduced trypanothione
-
 Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.8 90000
-
 x * 90000, SDS-PAGE Crithidia fasciculata
6.3.1.9 82000
-
 x * 82000, SDS-PAGE Crithidia fasciculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.8 gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP Crithidia fasciculata the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione ?
-
 ?
6.3.1.9 gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP Crithidia fasciculata the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.8 Crithidia fasciculata
-
-
-
6.3.1.9 Crithidia fasciculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.8
-
 Crithidia fasciculata
6.3.1.9
-
 Crithidia fasciculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.8 additional information
-
-
 Crithidia fasciculata
6.3.1.9 additional information
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.8 gamma-L-Glu-L-Cys-Gly + spermidine + ATP
-
 Crithidia fasciculata N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate
-
 ?
6.3.1.8 gamma-L-Glutamyl-L-cysteinyl-glycine + spermidine + ATP the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione Crithidia fasciculata ?
-
 ?
6.3.1.9 gamma-L-Glu-L-alpha-aminobutyrylglycine + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP gamma-L-Glu-L-alpha-aminobutyrylglycine i.e. ophthalmic acid Crithidia fasciculata ?
-
 ?
6.3.1.9 gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione Crithidia fasciculata N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
-
 ?
6.3.1.9 gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O
-
 Crithidia fasciculata N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.8 ? x * 90000, SDS-PAGE Crithidia fasciculata
6.3.1.8 More in vivo a complex between EC 6.3.1.8 and EC 6.3.1.9 exists that persists during purification Crithidia fasciculata
6.3.1.9 ? x * 82000, SDS-PAGE Crithidia fasciculata
6.3.1.9 More in vivo a complex between EC 6.3.1.8 and EC 6.3.1.9 exists that persists during purification Crithidia fasciculata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.8 additional information
-
 additional information
-
 Crithidia fasciculata
6.3.1.9 additional information
-
 additional information
-
 Crithidia fasciculata