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Literature summary extracted from

  • Winter, H.C.; Ti-Zhi Su; Dekker, E.E.
    4-Methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts (1983), Biochem. Biophys. Res. Commun., 111, 484-489.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.7 Asp with NH4+ is nitrogen donor Arachis hypogaea
6.3.1.7 Glu with NH4+ is nitrogen donor Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.7 8
-
 4-methylene-L-glutamate about Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.7 280000
-
 gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.7 ATP + 4-methylene-L-glutamate + NH3 Arachis hypogaea enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.7 Arachis hypogaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.7 partial Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.7 cotyledon
-
 Arachis hypogaea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.7 ATP + 4-methylene-L-glutamate + glutamine about 20% as effective as NH4+ Arachis hypogaea AMP + diphosphate + glutamate + 4-methylene-L-glutamine
-
 ?
6.3.1.7 ATP + 4-methylene-L-glutamate + hydroxylamine 70% as effective as NH4+ Arachis hypogaea AMP + diphosphate + 4-methylene-L-glutamylhydroxamate
-
 ?
6.3.1.7 ATP + 4-methylene-L-glutamate + NH3 enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts Arachis hypogaea ?
-
 ?
6.3.1.7 ATP + 4-methylene-L-glutamate + NH4+
-
 Arachis hypogaea AMP + diphosphate + 4-methylene-L-glutamine
-
 ?
6.3.1.7 additional information no amide/hydroxylamine exchange Arachis hypogaea ?
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.7 6.6 8.1 more than 70% of maximal activity at pH 6.6 and 8.1 Arachis hypogaea