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Literature summary extracted from

  • Khouri, H.E.; Vernet, T.; Menard, R.; Parlati, F.; Laflamme, P.; Tessier, D.C.; Gour-Salin, B.; Thomas, D.Y.; Storer, A.C.
    Engineering of papain (1991), Biochemistry, 30, 8929-8936.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.2 V133A/S205E change in specificity compared to wild-type enzyme Carica papaya
3.4.22.2 V133A/V157G/S205E important decrease in activity, change in specificity compared to the wild-type enzyme Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.089
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide
-
 Carica papaya
3.4.22.2 0.191
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/S205E Carica papaya
3.4.22.2 0.43
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya
3.4.22.2 0.94
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/S205E Carica papaya
3.4.22.2 1.296
-
 benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/S205E Carica papaya
3.4.22.2 1.48
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide
-
 Carica papaya
3.4.22.2 2.03
-
 benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya
3.4.22.2 2.27
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide
-
 Carica papaya
3.4.22.2 5.6
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide + H2O
-
 Carica papaya ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 0.42
-
 benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya
3.4.22.2 0.79
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya
3.4.22.2 1.18
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide
-
 Carica papaya
3.4.22.2 2.78
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya
3.4.22.2 5.67
-
 benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/S205E Carica papaya
3.4.22.2 8.14
-
 benzyloxycarbonyl-citrullyl-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/V157G/S205E Carica papaya
3.4.22.2 9.3
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/S205E Carica papaya
3.4.22.2 16.9
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide mutant enzyme V133A/S205E Carica papaya
3.4.22.2 41.6
-
 benzyloxycarbonyl-Phe-Arg 4-methylcoumarin-7-amide
-
 Carica papaya