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Literature summary extracted from
- Preparation and properties of immobilized papain and lipase (1977), Biotechnol. Bioeng., 14, 1703-1714.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.22.2 | immobilized enzyme does not lose any activity after tereatment with 6 M urea for 270 min, soluble papain loses 81% of its activity after urea treatment | Carica papaya |
| 3.4.22.2 | immobilized enzyme is stable at 4°C and pH 7.5 for up to 8 months, the soluble enzyme loses activity within 96 h | Carica papaya |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.2 | 0.0519 | - |
Nalpha-benzoyl-L-Arg ethyl ester | - |
Carica papaya |  |
| 3.4.22.2 | 0.0681 | - |
Nalpha-benzoyl-L-Arg ethyl ester | - |
Carica papaya | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.2 | Carica papaya | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.2 | casein + H2O | - |
Carica papaya | ? | - |
? | |
| 3.4.22.2 | Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Carica papaya | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.2 | 60 | - |
hydrolysis of casein, soluble and immobilized enzyme | Carica papaya |
| 3.4.22.2 | 65 | - |
hydrolysis of alpha-N-benzoyl-L-Arg ethyl ester, soluble enzyme | Carica papaya |
| 3.4.22.2 | 75 | - |
hydrolysis of alpha-N-benzoyl-L-Arg ethyl ester, immobilized enzyme | Carica papaya |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.2 | additional information | - |
- |
Carica papaya |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.2 | 6.5 | - |
hydrolysis of casein, soluble enzyme | Carica papaya |
| 3.4.22.2 | 7 | - |
hydrolysis of casein, immobilized enzyme | Carica papaya |
| 3.4.22.2 | 7.2 | - |
hydrolysis of alpha-N-benzoyl-L-Arg ethyl ester, soluble enzyme | Carica papaya |
| 3.4.22.2 | 7.5 | - |
optimal storage stability for the soluble and immobilize enzyme at 5°C | Carica papaya |
| 3.4.22.2 | 8.2 | - |
hydrolysis of alpha-N-benzoyl-L-Arg ethyl ester, immobilized enzyme | Carica papaya |
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Release 2023.1 (January 2023)
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