Literature summary extracted from

  • Storer, A.; Carey, P.R.
    Comparison of the kinetics and mechanism of the papain-catalyzed hydrolysis of esters and thiono esters (1985), Biochemistry, 24, 6808-6818.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.3
-
benzoyl-thiocarbamic acid methyl ester
-
Carica papaya
3.4.22.2 0.11
-
N-(beta-phenylpropionyl)Gly methyl thiono ester
-
Carica papaya
3.4.22.2 0.77
-
benzoyl-thiocarbamic acid
-
Carica papaya
3.4.22.2 16
-
benzoyl-thiocarbamic acid ethyl ester
-
Carica papaya
3.4.22.2 0.301
-
N-benzoyl-Gly methyl thiono ester
-
Carica papaya
3.4.22.2 6.63
-
N-(beta-phenylpropionyl)Gly methyl ester
-
Carica papaya
3.4.22.2 18.54
-
Nalpha-benzoyl-Gly methyl ester
-
Carica papaya
3.4.22.2 21.8
-
N-benzoyl-Gly methyl ester
-
Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 benzoyl-thiocarbamic acid + H2O
-
Carica papaya N-benzoyl-Gly + SH2
-
?
3.4.22.2 benzoyl-thiocarbamic acid ethyl ester + H2O
-
Carica papaya N-benzoyl-Gly + ethanethiol
-
?
3.4.22.2 benzoyl-thiocarbamic acid methyl ester + H2O
-
Carica papaya N-benzoyl thioglycine + methanol
-
?
3.4.22.2 N(beta-phenylpropionyl)Gly methyl ester + H2O
-
Carica papaya ?
-
?
3.4.22.2 N-(beta-phenylpropionyl)Gly methyl thiono ester + H2O
-
Carica papaya ?
-
?
3.4.22.2 N-benzoyl-Gly ethyl ester + H2O
-
Carica papaya N-benzoyl-Gly + ethanol
-
?
3.4.22.2 N-benzoyl-Gly methyl ester + H2O
-
Carica papaya ?
-
?
3.4.22.2 N-benzoyl-Gly methyl ester + H2O
-
Carica papaya N-benzoyl-Gly + methanol
-
?
3.4.22.2 N-benzoyl-Gly methyl thiono ester + H2O
-
Carica papaya ?
-
?
3.4.22.2 Nalpha-benzoyl-Gly methyl ester + H2O
-
Carica papaya Nalpha-benzoyl-Gly + methanol
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 0.064
-
benzoyl-thiocarbamic acid methyl ester
-
Carica papaya
3.4.22.2 3.4
-
benzoyl-thiocarbamic acid ethyl ester
-
Carica papaya
3.4.22.2 3.48
-
N-benzoyl-Gly methyl ester
-
Carica papaya
3.4.22.2 3340
-
benzoyl-thiocarbamic acid
-
Carica papaya