BRENDA - Enzyme Database

Purification of active Herpes simplex virus-1 protease expressed in Escherichia coli

Darke, P.L.; Chen, E.; Hall, D.L.; Sardanna, M.K.; Veloski, C.A.; LaFemina, R.L.; Shafer, J.A.; Kuo, L.C.; J. Biol. Chem. 269, 18708-18711 (1994) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.4.21.97
expression in Escherichia coli
Human alphaherpesvirus 1
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.97
0.88
-
HTYLQASEKFMW-NH2
-
Human alphaherpesvirus 1
3.4.21.97
1.36
-
HTYLQASEKFKM-NH2
-
Human alphaherpesvirus 1
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.21.97
Human alphaherpesvirus 1
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.21.97
-
Human alphaherpesvirus 1
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.21.97
ALVNASSAAHVDV-NH2 + H2O
-
95606
Human alphaherpesvirus 1
ALVNA + SSAAHVDV-NH2
-
95606
Human alphaherpesvirus 1
?
3.4.21.97
HTYLQASEKFKM-NH2 + H2O
-
95606
Human alphaherpesvirus 1
HTYLQA + SEKFKM-NH2
-
95606
Human alphaherpesvirus 1
?
3.4.21.97
HTYLQASEKFMW-NH2 + H2O
-
95606
Human alphaherpesvirus 1
HTYLQA + SEKFMW-NH2
-
95606
Human alphaherpesvirus 1
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.97
expression in Escherichia coli
Human alphaherpesvirus 1
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.97
0.88
-
HTYLQASEKFMW-NH2
-
Human alphaherpesvirus 1
3.4.21.97
1.36
-
HTYLQASEKFKM-NH2
-
Human alphaherpesvirus 1
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.97
-
Human alphaherpesvirus 1
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.21.97
ALVNASSAAHVDV-NH2 + H2O
-
95606
Human alphaherpesvirus 1
ALVNA + SSAAHVDV-NH2
-
95606
Human alphaherpesvirus 1
?
3.4.21.97
HTYLQASEKFKM-NH2 + H2O
-
95606
Human alphaherpesvirus 1
HTYLQA + SEKFKM-NH2
-
95606
Human alphaherpesvirus 1
?
3.4.21.97
HTYLQASEKFMW-NH2 + H2O
-
95606
Human alphaherpesvirus 1
HTYLQA + SEKFMW-NH2
-
95606
Human alphaherpesvirus 1
?