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Literature summary extracted from
- Bovine enterokinase. Purification, specificity, and some molecular properties (1977), Biochemistry, 16, 3354-3360.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.9 | 0.001 | - |
Trypsinogen | bovine guanidinated trypsinogen | Bos taurus |  |
| 3.4.21.9 | 0.007 | - |
Trypsinogen | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 57000 | - |
x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
| 3.4.21.9 | 82000 | - |
x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
| 3.4.21.9 | 145000 | - |
x * 145000, SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.9 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.9 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.9 | duodenum | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 1.36 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.9 | trypsinogen + H2O | cleaved exclusively at the Lys6-Ile7-peptide bond | Bos taurus | trypsin + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.9 | ? | x * 57000 + x * 82000, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
| 3.4.21.9 | ? | x * 145000, SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.9 | 60 | - |
complete destruction of activity towards trypsinogen after 2 min, 60% loss of esterase activity after 8 min. Presence of 4 mM CaCl2 stabilizes both activities | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.9 | 0.005 | - |
Trypsinogen | bovine guanidinated trypsinogen | Bos taurus | |
| 3.4.21.9 | 1.48 | - |
Trypsinogen | bovine trypsinogen | Bos taurus | |
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