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Literature summary extracted from
- Human plasminogen and plasmin (1970), Methods Enzymol., 19, 184-199.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.7 | freezing and thawing results in loss of activity | Homo sapiens |
| 3.4.21.7 | reduction with 2-mercaptoethanol in 8 M urea at pH 9.0, complete loss of activity by cleavage of a single disulfide bond | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.7 | 6-aminohexanoic acid | noncompetitive | Homo sapiens |  |
| 3.4.21.7 | alpha1 globulin | - |
Homo sapiens | |
| 3.4.21.7 | alpha2 globulin | - |
Homo sapiens | |
| 3.4.21.7 | aminomethyl cyclohexane carboxylic acid | - |
Homo sapiens | |
| 3.4.21.7 | human plasma protein inhibitors | - |
Homo sapiens | |
| 3.4.21.7 | Lima bean trypsin inhibitor | - |
Homo sapiens | |
| 3.4.21.7 | Pancreatic trypsin inhibitor | - |
Homo sapiens | |
| 3.4.21.7 | Soybean trypsin inhibitor | - |
Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.7 | 0.021 | - |
p-tosyl-Arg methyl ester | - |
Homo sapiens | |
| 3.4.21.7 | 0.03 | - |
fibrinogen | - |
Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.7 | 75400 | - |
equilibrium sedimentation | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.7 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.7 | proteolytic modification | mechanism of activation by urokinase, streptokinase, trypsin and pig heart activator is specific and proceeds primarily through the cleavage of a single Arg-Val bond | Homo sapiens |
| 3.4.21.7 | proteolytic modification | zymogen: plasminogen | Homo sapiens |
| 3.4.21.7 | side-chain modification | the enzyme contains less than 2% carbohydrate | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.7 | - |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.7 | -20°C to -30°C, pH 9.0, Tris-Lys-NaCl-EDTA buffer containing 25% glycerol, stable | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.7 | casein + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.7 | insulin + H2O | cleavage of the Arg25-Gly and Lys29-Ala peptide bonds of the beta-chain of oxidized bovine insulin | Homo sapiens | ? | - |
? | |
| 3.4.21.7 | protamin-heparin complex + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.7 | S-sulfo-fibrinogen + H2O | cleaves only Lys and Arg peptide bonds | Homo sapiens | ? | - |
? | |
| 3.4.21.7 | Tosyl-Arg methyl ester + H2O | p-tosyl-Arg methyl ester | Homo sapiens | ? | - |
? | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.7 | 4 | - |
very stable below | Homo sapiens |
| 3.4.21.7 | 7 | - |
unstable in absence of glycerol | Homo sapiens |
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Release 2023.1 (January 2023)
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