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Literature summary extracted from

  • Fenton, J.W.; Fasco, M.J.; Stackrow, A.B.; Aronson, D.L.; Young, A.M.; Finlayson, J.S
    Human thrombins. Production, evaluation, and properties of alpha-thrombin (1977), J. Biol. Chem., 252, 3587-3598.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.5 autolytic degradation during sedimentation in neutral salt solution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.5 diisopropyl fluorophosphate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.5 31500 40600 thrombin in 0.21 M NaCl, 0.02 M sodium phosphate, pH 7.2, equilibrium sedimentation Homo sapiens
3.4.21.5 32000
-
 1 * 4600, A chain, + 1 * 32000, B chain, reduced alpha-thrombin, SDS-PAGE Homo sapiens
3.4.21.5 36600
-
 equilibrium sedimentation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.5 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.5 side-chain modification carbohydrate is associated only with the B chain Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.21.5 -50°C, 0.75 M NaCl, pH 6, stable for about 1 year Homo sapiens
3.4.21.5 4°C, 0.75 M NaCl, pH 6, stable for about 1 week Homo sapiens

Subunits

EC Number Subunits Comment Organism
3.4.21.5 dimer 1 * 4600, A chain, + 1 * 32000, B chain, reduced alpha-thrombin, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.5 23
-
 stable for up to 3 days at room temperature Homo sapiens
3.4.21.5 35
-
 pH 6, loss of activity above Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.5 5 10.3 rapid loss of clotting and esterase activity below pH 5 and above pH 10.3 Homo sapiens