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Literature summary extracted from

  • Landis, B.H.; Koehler, K.A.; Fenton II, J.W.
    Human thrombins. Group IA and IIA salt-dependent properties of alpha-thrombin (1981), J. Biol. Chem., 256, 4604-4610.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.5 autolytic degradation of alpha-thrombin to nonclotting beta-thrombins and gamma-thrombins is retarded by increasing concentrations of Li+, Cs+, K+, Rb+ chlorides Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.5 0.0052
-
 benzoyl-Arg ethyl ester 0.15 M KCl Homo sapiens
3.4.21.5 0.0056
-
 benzoyl-Arg ethyl ester 0.15 M NaCl Homo sapiens
3.4.21.5 0.0057
-
 benzoyl-Arg ethyl ester 0.15 M CsCl Homo sapiens
3.4.21.5 0.0063
-
 tosyl-Arg methyl ester 0.15 M NaCl Homo sapiens
3.4.21.5 0.007
-
 benzoyl-Arg ethyl ester 0.15 M NaNO3 Homo sapiens
3.4.21.5 0.0072
-
 tosyl-Arg methyl ester 0.15 M NaNO3 Homo sapiens
3.4.21.5 0.0075
-
 benzoyl-Arg methyl ester 0.15 M KCl Homo sapiens
3.4.21.5 0.0079
-
 benzoyl-Arg ethyl ester 0.15 M CsNO3 Homo sapiens
3.4.21.5 0.0088
-
 benzoyl-Arg methyl ester 0.15 M NaCl Homo sapiens
3.4.21.5 0.0122
-
 tosyl-Arg methyl ester 0.15 M KCl Homo sapiens
3.4.21.5 0.0133
-
 tosyl-Arg methyl ester 0.15 M CsNO3 Homo sapiens
3.4.21.5 0.0137
-
 tosyl-Arg methyl ester 0.15 M RbCl Homo sapiens
3.4.21.5 0.0152
-
 tosyl-Arg methyl ester 0.15 M CsCl Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.5 Fibrinogen + H2O Homo sapiens clotting of fibrinogen ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.5 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.5 benzoyl-Arg ethyl ester + H2O
-
 Homo sapiens benzoyl-Arg + ethanol
-
 ?
3.4.21.5 benzoyl-Arg methyl ester + H2O
-
 Homo sapiens benzoyl-Arg + methanol
-
 ?
3.4.21.5 Fibrinogen + H2O clotting of fibrinogen Homo sapiens ?
-
 ?
3.4.21.5 tosyl-Arg ethyl ester + H2O
-
 Homo sapiens tosyl-Arg + ethanol
-
 ?
3.4.21.5 Tosyl-Arg methyl ester + H2O
-
 Homo sapiens Tosyl-Arg + methanol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.5 additional information
-
 with increasing salt concentrations, the thermal stability of the enzyme activity is improved by group Ia clorides in decreasing order: Na+, K+, Rb+, Cs+. For ionic strength below 3 M the stability is reduced by group IIa chlorides in increasoing order: Mg2+, Sr2+, Ba2+, Ca2+. If the ionic strenght is about 3 M, the stability changes only slightly in LiCl or NaNO3 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.5 3.27
-
 tosyl-Arg ethyl ester 0.15 M NaCl Homo sapiens
3.4.21.5 3.78
-
 tosyl-Arg ethyl ester 0.15 M NaNO3 Homo sapiens
3.4.21.5 9.33
-
 tosyl-Arg ethyl ester 0.15 M RbCl Homo sapiens
3.4.21.5 9.4
-
 tosyl-Arg ethyl ester 0.15 M KCl Homo sapiens
3.4.21.5 10
-
 tosyl-Arg ethyl ester 0.15 M CsNO3 Homo sapiens
3.4.21.5 10.3
-
 tosyl-Arg ethyl ester 0.15 M CsCl Homo sapiens
3.4.21.5 13.7
-
 benzoyl-Arg ethyl ester 0.15 M NaNO3 Homo sapiens
3.4.21.5 14
-
 benzoyl-Arg methyl ester 0.15 M NaCl Homo sapiens
3.4.21.5 14.1
-
 benzoyl-Arg ethyl ester 0.15 M NaCl Homo sapiens
3.4.21.5 15
-
 benzoyl-Arg methyl ester 0.15 M KCl Homo sapiens
3.4.21.5 15.2
-
 benzoyl-Arg ethyl ester 0.15 M KCl Homo sapiens
3.4.21.5 16.1
-
 benzoyl-Arg ethyl ester 0.15 M CsCl Homo sapiens
3.4.21.5 17.8
-
 benzoyl-Arg ethyl ester 0.15 M CsNO3 Homo sapiens