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Literature summary extracted from

  • Lenz, R.; Zenk, M.H.
    Purification and properties of codeinone reductase (NADPH) from Papaver somniferum cell cultures and differentiated plants (1995), Eur. J. Biochem., 233, 132-139.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.247 0.009
-
 codeinone isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.023
-
 codeinone enzyme from cell culture Papaver somniferum
1.1.1.247 0.026
-
 Hydrocodone isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.027
-
 Hydrocodone enzyme from cell culture Papaver somniferum
1.1.1.247 0.027
-
 Hydrocodone morphine, enzyme from cell culture Papaver somniferum
1.1.1.247 0.029
-
 NADP+ enzyme from cell culture Papaver somniferum
1.1.1.247 0.042
-
 Hydromorphone isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.047
-
 NADP+ isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.05
-
 Hydromorphone enzyme from cell culture Papaver somniferum
1.1.1.247 0.063
-
 morphine isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.076
-
 codeine enzyme from cell culture Papaver somniferum
1.1.1.247 0.081
-
 NADPH isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.168
-
 NADPH enzyme from cell culture Papaver somniferum
1.1.1.247 0.178
-
 codeine isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.422
-
 Dihydrocodeine isoenzymes from capsule tissue Papaver somniferum
1.1.1.247 0.424
-
 Dihydrocodeine enzyme from cell culture Papaver somniferum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.247 cytosol
-
 Papaver somniferum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.247 35000
-
 gel filtration Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.247 Codeinone + NADPH Papaver somniferum biosynthesis of codeine Codeine + NADP+
-
 ?
1.1.1.247 Morphinone + NADPH Papaver somniferum biosynthesis of morphine Morphine + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.247 Papaver somniferum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.247 2 isoenzymes from capsule tissue, enzyme from cell culture Papaver somniferum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.247 cell culture
-
 Papaver somniferum
-
1.1.1.247 leaf
-
 Papaver somniferum
-
1.1.1.247 plant capsule
-
 Papaver somniferum
-
1.1.1.247 root
-
 Papaver somniferum
-
1.1.1.247 shoot
-
 Papaver somniferum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.247 additional information
-
-
 Papaver somniferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.247 Codeinone + NADPH r Papaver somniferum Codeine + NADP+ (-)codeine ?
1.1.1.247 Codeinone + NADPH (-)codeinone Papaver somniferum Codeine + NADP+ (-)codeine ?
1.1.1.247 Codeinone + NADPH no reaction with NADH and NAD+ Papaver somniferum Codeine + NADP+ (-)codeine ?
1.1.1.247 Codeinone + NADPH biosynthesis of codeine Papaver somniferum Codeine + NADP+
-
 ?
1.1.1.247 Dihydrocodeine + NADP+
-
 Papaver somniferum ?
-
 ?
1.1.1.247 Hydrocodone + NADPH
-
 Papaver somniferum ?
-
 ?
1.1.1.247 Hydromorphone + NADPH
-
 Papaver somniferum ?
-
 ?
1.1.1.247 Morphinone + NADPH
-
 Papaver somniferum Morphine + NADP+
-
 ?
1.1.1.247 Morphinone + NADPH biosynthesis of morphine Papaver somniferum Morphine + NADP+
-
 ?
1.1.1.247 Naloxone + NADPH
-
 Papaver somniferum ?
-
 ?
1.1.1.247 Naltrexone + NADPH
-
 Papaver somniferum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.247 monomer 34000-35000, SDS-PAGE Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.247 40
-
 reduction of codeinone Papaver somniferum
1.1.1.247 46
-
 oxidation of codeine Papaver somniferum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.247 29 47 50% of maximal activity at 29°C and 47°C, reduction of codeinone Papaver somniferum
1.1.1.247 34 53 50% of maximal activity at 34°C and 53°C, oxidation of codeine Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.247 7
-
 reduction of codeinone Papaver somniferum
1.1.1.247 9.1
-
 oxidation of codeine Papaver somniferum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.247 6.2 7.6 50% of maximal activity at pH 6.2 and pH 7.6, reduction of codeinone Papaver somniferum
1.1.1.247 8.2 9.8 50% of maximal activity at pH 8.2 and pH 9.8, oxidation of codeine Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.247 NADP+
-
 Papaver somniferum
1.1.1.247 NADPH
-
 Papaver somniferum