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Literature summary extracted from

  • Olafson, R.W.; Smilie, L.B.
    Enzymic and physicochemical properties of Streptomyces griseus trypsin (1975), Biochemistry, 14, 1161-1167.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.4 significant stabilization in the neutral and alkaline range by 15 mM Ca2+ Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 L-1-chloro-3-tosylamido-4-phenyl-2-butanone
-
 Streptomyces griseus
3.4.21.4 p-tosyl-L-Lys-chloromethane reaction towards B chain of insulin is inhibited, the activity towards the A-chain is not significantly affected Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.0077
-
 N-benzoyl-L-Arg ethyl ester
-
 Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 22800
-
 low-speed equilibrium sedimentation Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Streptomyces griseus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.21.4 reversible denaturation at pH 12.0 and at pH 2.0 Streptomyces griseus

Storage Stability

EC Number Storage Stability Organism
3.4.21.4 0°C, pH 2-10, stable Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 insulin + H2O oxidized A chain, and B chain Streptomyces griseus ?
-
 ?
3.4.21.4 N-Benzoyl-L-Arg ethyl ester + H2O
-
 Streptomyces griseus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 30
-
 maximal stability at pH 3-4 Streptomyces griseus
3.4.21.4 30
-
 pH 8.0, 75% loss of activity after 25 min, 90% loss of activity after 24 h Streptomyces griseus
3.4.21.4 30
-
 pH 10.0, complete loss of activitry after 25 min Streptomyces griseus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.4 additional information
-
 significant stabilization in the neutral and alkaline range by 15 mM Ca2+ Streptomyces griseus
3.4.21.4 1
-
 0°C, 12 h, 20% loss of activity Streptomyces griseus
3.4.21.4 2 10 0°C, very stable Streptomyces griseus
3.4.21.4 10
-
 20% loss of activity after 12 h at 0°C, complete loss of activity after 25 min at 30°C Streptomyces griseus