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Literature summary extracted from

  • Asgeirsson, B.; Fox, J.W.; Bjarnason, J.B.
    Purification and characterization of trypsin from the poikilotherm Gadus morhua (1989), Eur. J. Biochem., 180, 85-94.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 ovomucoid
-
 Gadus morhua
3.4.21.4 p-tosyl-L-Lys-chloromethane
-
 Gadus morhua
3.4.21.4 phenylmethyl sulfonyl fluoride
-
 Gadus morhua
3.4.21.4 Soybean trypsin inhibitor
-
 Gadus morhua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.029
-
 p-tosyl-L-Arg methyl ester
-
 Gadus morhua
3.4.21.4 0.077
-
 Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
 Gadus morhua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 23200
-
 x * 23200, SDS-PAGE Gadus morhua
3.4.21.4 24700
-
 gel filtration Gadus morhua

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Gadus morhua
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4
-
 Gadus morhua

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 cecum
-
 Gadus morhua
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 142.8
-
 enzyme II Gadus morhua
3.4.21.4 155.7
-
 enzyme III Gadus morhua
3.4.21.4 339
-
 enzyme I Gadus morhua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O Nalpha-benzoyl-L-Arg-p-nitroanilide Gadus morhua ?
-
 ?
3.4.21.4 p-tosyl-L-Arg methyl ester + H2O
-
 Gadus morhua ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.4 ? x * 23200, SDS-PAGE Gadus morhua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 8
-
-
 Gadus morhua

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 80% of maximal activity Gadus morhua

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.4 2
-
 30 min, 5°C, about 55% loss of maximal activity Gadus morhua
3.4.21.4 4
-
 30 min, 5°C, about 15% loss of maximal activity Gadus morhua
3.4.21.4 5 7 5°C, highest stability Gadus morhua
3.4.21.4 10
-
 30 min, 5°C, 15% loss of activity Gadus morhua