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Literature summary extracted from

  • O'Connor, B.; O'Cuinn, G.
    Purification of and kinetic studies on a narrow specificity synaptosomal membrane pyroglutamate aminopeptidase from guinea-pig brain (1985), Eur. J. Biochem., 150, 47-52.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.19.6 inclusion of 50% glycerol prevents most of the harmful effect of freezing in imidazole buffer Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 Acid thyroliberin competitive inhibition of thyroliberin hydrolysis Cavia porcellus
3.4.19.3 Anorexogenic peptide competitive inhibition of thyroliberin hydrolysis Cavia porcellus
3.4.19.3 bradykinin-potentiating peptide 5a competitive inhibition of thyroliberin hydrolysis Cavia porcellus
3.4.19.3 Eledoisin competitive inhibition of thyroliberin hydrolysis Cavia porcellus
3.4.19.3 Luliberin competitive inhibition of thyroliberin hydrolysis Cavia porcellus
3.4.19.3 neurotensin competitive inhibition of thyroliberin hydrolysis Cavia porcellus
3.4.19.6 Luliberin inhibits hydrolysis of thyroliberin competitively Cavia porcellus
3.4.19.6 Peptides containing N-terminal pyroglutamyl residues
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.3 0.04
-
 Thyroliberin
-
 Cavia porcellus
3.4.19.6 0.04
-
 Thyroliberin
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.19.3 membrane synaptosomal Cavia porcellus 16020
-
3.4.19.6 synaptosome
-
 Cavia porcellus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Cavia porcellus
-
-
-
3.4.19.6 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3
-
 Cavia porcellus
3.4.19.6
-
 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.3 brain
-
 Cavia porcellus
-
3.4.19.6 brain
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.3 0.323
-
-
 Cavia porcellus
3.4.19.6 0.3237
-
-
 Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
3.4.19.6 -20°C, potassium phosphate buffer, pH 7.4, stable for 6 months Cavia porcellus
3.4.19.6 15°C, 5% loss of activity after 7 days Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 5-pyrrolidone-2-carboxyl-His-Gly + H2O slight activity Cavia porcellus ?
-
 ?
3.4.19.3 5-pyrrolidone-2-carboxyl-His-Trp + H2O
-
 Cavia porcellus ?
-
 ?
3.4.19.3 acid-thyroliberin + H2O
-
 Cavia porcellus pyroglutamate + His-Pro
-
 ?
3.4.19.3 additional information no hydrolysis of dipeptides containing N-terminal pyroglutamic acid or with pyroglutamyl peptides containing more than three amino acids Cavia porcellus ?
-
 ?
3.4.19.3 thyroliberin + H2O
-
 Cavia porcellus pyroglutamate + His-Pro-NH2
-
 ?
3.4.19.6 acid-thyroliberin + H2O
-
 Cavia porcellus ?
-
 ?
3.4.19.6 luliberin N-terminal tripeptide + H2O pyroglutamyl-His-Trp Cavia porcellus ?
-
 ?
3.4.19.6 additional information no hydrolysis of pyroglutamyl peptides containing more than three amino acids Cavia porcellus ?
-
 ?
3.4.19.6 additional information no hydrolysis of dipeptides containing N-terminal pyroglutamic acid Cavia porcellus ?
-
 ?
3.4.19.6 pyroglutamyl-His-Gly + H2O slight Cavia porcellus pyroglutamate + His-Gly
-
 ?
3.4.19.6 thyroliberin + H2O
-
 Cavia porcellus pyroglutamic acid + His-Pro-NH2
-
 ?